Somatic embryogenesis of rose (Rosa sp.)

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dc.contributor.advisor Dong, Zhongmin, 1957-
dc.creator Estabrooks, Tammy Lynn, 1974-
dc.date.accessioned 2011-05-09T12:32:22Z
dc.date.available 2011-05-09T12:32:22Z
dc.date.issued 2004
dc.identifier.other SB411.54 E88 2004
dc.identifier.uri http://library2.smu.ca/xmlui/handle/01/22584
dc.description xii, 151 leaves : ill. (some col.) ; 28 cm.
dc.description Includes abstract.
dc.description Includes bibliographical references (leaves 130-140).
dc.description.abstract Somatic embryogenesis (SE) offers vast potential as a method for clonal propagation of roses. By this method, tissue from an elite plant is cultured in vitro and induced into an embryogenic state, in which bipolar somatic embryos are formed. The somatic embryos are capable of regenerating whole plants that are genetically identical to the plant used as the original tissue source. Although somatic embryogenesis holds several advantages over conventional means of propagation, SE technology requires improvement before use on a commercial scale. This thesis describes studies aimed at developing somatic embryogenesis technology for roses. A suitable methodology for SE of the commercially valuable rose cultivar 'Livin' Easy' ( Rosa hybrida L.) was developed. This is the first report of SE induction in the cultivar 'Livin' Easy'. Murashige and Skoog basal medium supported growth and promoted successful induction of embryogenic tissue when supplemented with plant growth regulators, whereas Woody Plant Medium and plant growth regulator-free medium did not. The synthetic auxin 2,4,5-trichlorophenoxyacetic acid was successful in inducing SE at higher rates and over a greater concentration range than the commonly employed 2,4-dichlorophenoxyacetic acid. This was the first report of the utilization of 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) for the induction of SE in rose. Somatic embryos successfully converted into plantlets at high rates up to 95%. Embryogenic tissue occurred on 60% of the plantlets, a phenomenon referred to as recurrent somatic embryogenesis.
dc.language.iso en
dc.publisher Halifax, N.S. : Saint Mary's University
dc.subject.lcc SB411.54
dc.subject.lcsh Roses -- Somatic embryogenesis
dc.subject.lcsh Embryology
dc.subject.lcsh Rose culture
dc.title Somatic embryogenesis of rose (Rosa sp.)
dc.type Text
thesis.degree.name Master of Science in Applied Science
thesis.degree.level Masters
thesis.degree.discipline Biology
thesis.degree.grantor Saint Mary's University (Halifax, N.S.)


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