SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley

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dc.creator Ren, Xifeng
dc.creator Wang, Jibin
dc.creator Liu, Lipan
dc.creator Sun, Genlou
dc.creator Li, Chengdao
dc.creator Luo, Hong
dc.creator Sun, Dongfa 2018-02-06T15:53:10Z 2018-02-06T15:53:10Z 2016-08-17
dc.identifier.issn 2045-2322
dc.description Publisher's Version/PDF
dc.description.abstract A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley. en_CA
dc.language.iso en en_CA
dc.publisher Nature Publishing Group en_CA
dc.rights Creative Commons Attribution License 4.0
dc.subject.lcsh Barley -- Genetics
dc.subject.lcsh Dwarfism in plants
dc.title SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley en_CA
dc.type Text en_CA
dcterms.bibliographicCitation Scientific Reports 6, 31741. (2016) DOI: 10.1038/srep31741 en_CA

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