Distinct origin of the Y and St Genome in Elymus species: evidence from the analysis of a large sample of St genome species using two nuclear genes

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dc.creator Yan, Chi
dc.creator Sun, Genlou
dc.creator Sun, Dongfa
dc.date.accessioned 2018-06-12T12:53:21Z
dc.date.available 2018-06-12T12:53:21Z
dc.date.issued 2011-10-27
dc.identifier.issn 1932-6203
dc.identifier.uri http://library2.smu.ca/handle/01/27551
dc.description Publisher's Version/PDF
dc.description.abstract Background: Previous cytological and single copy nuclear genes data suggested the St and Y genome in the StY-genomic Elymus species originated from different donors: the St from a diploid species in Pseudoroegneria and the Y from an unknown diploid species, which are now extinct or undiscovered. However, ITS data suggested that the Y and St genome shared the same progenitor although rather few St genome species were studied. In a recent analysis of many samples of St genome species Pseudoroegneria spicata (Pursh) À. Love suggested that one accession of P. spicata species was the most likely donor of the Y genome. The present study tested whether intraspecific variation during sampling could affect the outcome of analyses to determining the origin of Y genome in allotetraploid StY species. We also explored the evolutionary dynamics of these species. Methodology/Principal Findings: Two single copy nuclear genes, the second largest subunit of RNA polymerase II (RPB2) and the translation elongation factor G (EF-G) sequences from 58 accessions of Pseudoroegneria and Elymus species, together with those from Hordeum (H), Agropyron (P), Australopyrum (W), Lophopyrum (E<sup>e</sup>), Thinopyrum (E<sup>a</sup>), Thinopyrum (E<sup>b</sup>), and Dasypyrum (V) were analyzed using maximum parsimony, maximum likelihood and Bayesian methods. Sequence comparisons among all these genomes revealed that the St and Y genomes are relatively dissimilar. Extensive sequence variations have been detected not only between the sequences from St and Y genome, but also among the sequences from diploid St genome species. Phylogenetic analyses separated the Y sequences from the St sequences. Conclusions/Significance: Our results confirmed that St and Y genome in Elymus species have originated from different donors, and demonstrated that intraspecific variation does not affect the identification of genome origin in polyploids. Moreover, sequence data showed evidence to support the suggestion of the genome convergent evolution in allopolyploid StY genome species. en_CA
dc.language.iso en en_CA
dc.publisher Public Library of Science en_CA
dc.rights Creative Commons Attribution License 4.0
dc.subject.lcsh Elymus -- Genetics
dc.subject.lcsh Elymus -- Genome mapping
dc.subject.lcsh Genomics
dc.title Distinct origin of the Y and St Genome in Elymus species: evidence from the analysis of a large sample of St genome species using two nuclear genes
dc.type Text en_CA
dcterms.bibliographicCitation Plos One 6(10), e26853. (2011) en_CA


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