dc.contributor.advisor |
Dong, Zhongmin, 1957- |
|
dc.creator |
Zou, Yinan |
|
dc.date.accessioned |
2013-09-05T15:14:44Z |
|
dc.date.available |
2013-09-05T15:14:44Z |
|
dc.date.issued |
2012 |
|
dc.identifier.other |
QR111 Z68 2012 |
|
dc.identifier.uri |
http://library2.smu.ca/xmlui/handle/01/25126 |
|
dc.description |
146 leaves : ill. (some col.) ; 29 cm. |
en_CA |
dc.description |
Includes abstract. |
|
dc.description |
Includes bibliographical references (leaves 139-146). |
|
dc.description.abstract |
Previous studies have showed that H[subscript 2] evolved from HUP- legume nodules is oxidized by rhizospheric microorganisms and alters the soil microbial diversity in rhizosphere. This H[subscript 2] oxidation is linked with the CO[subscript 2] fixation and O[subscript 2] reduction activities. Metatranscriptome analysis in this study revealed that soil bacterial community was changed significantly by H[subscript 2] . Populations of [beta]-proteobacteria and [delta]-proteobacteria were stimulated by H[subscript 2] exposure whereas Actinobacteria was suppressed. Results also showed that genes related with CO[subscript 2] fixation and O[subscript 2] reduction were up-regulated after H[subscript 2] treatment. Several speculated pathways were generated for the H[subscript 2] metabolisms in the soil. The quantification analysis of hydrogenase, the key enzyme in H[subscript 2] oxidation in soil, was carried out using HoxK NiFe hydrogenase small subunit gene via real time PCR. The result showed that the gene copy number had a significant increase after H[subscript 2] treatment. |
en_CA |
dc.description.provenance |
Submitted by Dianne MacPhee (dianne.macphee@smu.ca) on 2013-09-05T15:14:44Z
No. of bitstreams: 0 |
en |
dc.description.provenance |
Made available in DSpace on 2013-09-05T15:14:44Z (GMT). No. of bitstreams: 0
Previous issue date: 2012 |
en |
dc.language.iso |
en |
en_CA |
dc.publisher |
Halifax, N.S. : Saint Mary's University, |
en_CA |
dc.subject.lcc |
QR111 |
|
dc.subject.lcsh |
Soil microbiology |
|
dc.subject.lcsh |
Gene expression |
|
dc.subject.lcsh |
Hydrogen |
|
dc.title |
Effect of H₂ on soil bacterial community structure and gene expression |
en_CA |
dc.title.alternative |
Effect of H[subscript 2] on soil bacterial community structure and gene expression |
en_CA |
dc.title.alternative |
Effect of hydrogen on soil bacterial community structure and gene expression |
en_CA |
dc.type |
Text |
en_CA |
thesis.degree.level |
Masters |
|
thesis.degree.level |
Master of Science in Applied Science |
|
thesis.degree.discipline |
Biology |
|
thesis.degree.grantor |
Saint Mary's University (Halifax, N.S.) |
|