dc.contributor.advisor |
Dong, Zhongmin, 1957- |
|
dc.creator |
Maimaiti, Jiamila |
|
dc.date.accessioned |
2011-05-09T12:31:56Z |
|
dc.date.available |
2011-05-09T12:31:56Z |
|
dc.date.issued |
2005 |
|
dc.identifier.other |
QR113 M35 2005 |
|
dc.identifier.uri |
http://library2.smu.ca/xmlui/handle/01/22356 |
|
dc.description |
x, 97 leaves : ill. (some col.) ; 28 cm. |
|
dc.description |
Includes abstract and appendices. |
|
dc.description |
Includes bibliographical references (leaves 78-87). |
|
dc.description.abstract |
Previous studies have shown that the hydrogen (H₂) gas evolved from legume nodules inoculated by hydrogen releasing (Hup⁻) rhizobium strains contributes to growth promotion of subsequent crop plants by altering the populations of soil H₂ oxidizing bacteria; this may be responsible for the major beneficial effect of legumes in crop rotation. To further study the plant growth promoting bacteria in H₂ treated soil and soil adjacent to the Hup⁻ legume nodules, the H₂ oxidizing bacteria were isolated from H₂ treated soil and soil adjacent to Hup⁻ soybean nodules grown in greenhouse and field. A much higher bacterial density was found in soils with a higher H₂ uptake rate. Twenty isolates of H₂ oxidizing bacteria were obtained. They are slow growing bacteria on mineral salt medium incubated under H₂- enriched air. All H₂ oxidizing bacterial strains exhibited one high K[subscript m] for H₂ at 1000ppm. The isolates of H₂ oxidizing bacteria were identified as Variovorax paradoxus, Flavobacterium johnsonae and Burkholderia sordidicola by conventional microbiological tests and 16S rRNA gene sequence analysis. All isolated H₂ oxidizing bacteria increased the root elongation of spring wheat seedlings ranging from 21% to 254% compared to controls. Seventeen of them had a significantly positive effect on root elongation (p<0.005). The strains of V. paradoxus found in both H₂ treated soil and soil adjacent to Hup⁻ soybean nodules had the highest direct plant growth effect. The results confirm the hypothesis that the microorganisms responsible for the oxidation of H₂ released by Hup⁻ legume nodules are bacteria and they act as plant growth promoting rhizobacteria (PGPR). |
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dc.description.provenance |
Made available in DSpace on 2011-05-09T12:31:56Z (GMT). No. of bitstreams: 0 |
en |
dc.language.iso |
en |
|
dc.publisher |
Halifax, N.S. : Saint Mary's University |
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dc.subject.lcc |
QR113 |
|
dc.subject.lcsh |
Bacteria -- Identification |
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dc.subject.lcsh |
Rhizobium |
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dc.subject.lcsh |
Microbial biotechnology |
|
dc.subject.lcsh |
Plant growth-promoting rhizobacteria |
|
dc.subject.lcsh |
Soil microbiology |
|
dc.title |
Isolation and characterization of H₂ oxidizing bacteria in H₂ treated soil and soil adjacent to Hup⁻ soybean nodules |
|
dc.type |
Text |
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thesis.degree.name |
Master of Science in Applied Science |
|
thesis.degree.level |
Masters |
|
thesis.degree.discipline |
Biology |
|
thesis.degree.grantor |
Saint Mary's University (Halifax, N.S.) |
|