Development of an electrochemical-surface enhanced Raman spectroscopic (EC-SERS) sensor for bacterial screening

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dc.contributor.advisor Brosseau, Christa L.
dc.contributor.advisor Sit, Clarissa
dc.creator Lynk, Taylor Paige
dc.date.accessioned 2019-06-13T14:49:19Z
dc.date.available 2019-06-13T14:49:19Z
dc.date.issued 2018
dc.identifier.uri http://library2.smu.ca/handle/01/28923
dc.description.abstract The lack of efficiency of current methods for bacterial screening has prompted an increasing interest in developing a cost-effective, rapid and sensitive alternative for applications in all sectors of society. This project aims to explore a new detection platform for bacterial screening by coupling an applied electric potential with surface-enhanced Raman spectroscopy (SERS), a technique termed electrochemical surface-enhanced Raman spectroscopy (EC-SERS). The goal of using this technique is to improve upon the sensitivity and reproducibility of normal SERS to allow for rapid, point-of-need (PON) bacterial detection and identification. This project first shows the EC-SERS characterization of the commonly observed nucleotide breakdown products that dominate the SERS spectra of bacteria: adenine, guanine, xanthine, hypoxanthine, uric acid, 5’-adenosine monophosphate (AMP), and guanosine. This thesis work then concentrates on developing a sample preparation method to be used to study bacteria using EC-SERS for the first time. The results of this project demonstrate the improvement of the SERS spectra of both E. coli K-12 and B. megaterium bacteria obtained when an electric potential is employed, and highlights the great promise of EC-SERS for use as a fast and affordable bacterial screening method. en_CA
dc.description.provenance Submitted by Greg Hilliard (greg.hilliard@smu.ca) on 2019-06-13T14:49:19Z No. of bitstreams: 1 Lynk_Taylor_Honours_2018.pdf: 5951287 bytes, checksum: da024f8b6d3770f20e6ec73422f2dfb8 (MD5) en
dc.description.provenance Made available in DSpace on 2019-06-13T14:49:19Z (GMT). No. of bitstreams: 1 Lynk_Taylor_Honours_2018.pdf: 5951287 bytes, checksum: da024f8b6d3770f20e6ec73422f2dfb8 (MD5) Previous issue date: 2018-04-20 en
dc.language.iso en en_CA
dc.publisher Halifax, N.S. : Saint Mary's University
dc.title Development of an electrochemical-surface enhanced Raman spectroscopic (EC-SERS) sensor for bacterial screening en_CA
dc.type Text en_CA
thesis.degree.name Bachelor of Science (Honours Chemistry)
thesis.degree.level Undergraduate
thesis.degree.discipline Chemistry
thesis.degree.grantor Saint Mary's University (Halifax, N.S.)
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