dc.contributor.advisor |
Dong, Zhongmin, 1957- |
|
dc.creator |
He, Xiang (Nancy) |
|
dc.date.accessioned |
2011-02-10T19:13:51Z |
|
dc.date.available |
2011-02-10T19:13:51Z |
|
dc.date.issued |
2010 |
|
dc.identifier.other |
QR111 H455 2010 |
|
dc.identifier.uri |
http://library2.smu.ca/xmlui/handle/01/21933 |
|
dc.description |
148 leaves : ill. ; 29 cm. |
en_CA |
dc.description |
Includes bibliographical references (leaves 139-148) |
|
dc.description |
Includes abstract |
|
dc.description.abstract |
Previous studies have shown that H₂ from HUPˉ legume nodules promotes plant growth and enhances N₂O emission from soil, possibly as the result of hydrogen-induced variation of rhizobacterial community structure. Using terminal restriction fragment length polymorphism and 16S rRNA clone library, the current study revealed that the major bacterial groups that responded to H₂ exposure belong to Gammaproteobacteria and Bacteroidetes . To understand the relationship between H₂ exposure and soil denitrification, the gene copy number of a key gene in the denitrification process, nirK , encoding copper nitrite reductase, was quantified in soil samples with different treatments using a real-time PCR technique. The result showed that the nirK gene copy numbers in soil increased significant after H₂ treatment. Several key genes in the denitrification pathway (such as qnorB, nosZ ) were confirmed in some soil H₂-oxidizing bacterial isolates suggesting a connection between H₂ uptake and N₂O emission. |
en_CA |
dc.description.provenance |
Submitted by Dianne MacPhee (dianne.macphee@smu.ca) on 2011-02-10T19:13:51Z
No. of bitstreams: 0 |
en |
dc.description.provenance |
Made available in DSpace on 2011-02-10T19:13:51Z (GMT). No. of bitstreams: 0
Previous issue date: 2010 |
en |
dc.language.iso |
en |
en_CA |
dc.publisher |
Halifax, N.S. : Saint Mary's University |
en_CA |
dc.subject.lcc |
QR111 |
|
dc.subject.lcsh |
Soil microbiology |
|
dc.subject.lcsh |
Nitrogen |
|
dc.subject.lcsh |
Molecular microbiology |
|
dc.title |
Effect of H₂ on soil bacterial community structure and soil denitrifier population |
en_CA |
dc.title.alternative |
Effect of H[subscript 2] on soil bacterial community structure and soil denitrifier population |
en_CA |
dc.type |
Text |
|
thesis.degree.name |
Master of Science in Applied Science |
|
thesis.degree.level |
Masters |
|
thesis.degree.discipline |
Biology |
|
thesis.degree.grantor |
Saint Mary's University (Halifax, N.S.) |
|