Genome constitution and evolution of Elytrigia lolioides inferred from Acc1, EF-G, ITS, TrnL-F sequences and GISH

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dc.creator Wang, Long
dc.creator Jiang, Yuanyuan
dc.creator Shi, Qinghua
dc.creator Wang, Yi
dc.creator Sha, Lina
dc.creator Fan, Xing
dc.creator Kang, Houyang
dc.creator Zhang, Haiqin
dc.creator Sun, Genlou
dc.creator Zhang, Li
dc.creator Zhou, Yonghong
dc.date.accessioned 2021-09-29T16:15:39Z
dc.date.available 2021-09-29T16:15:39Z
dc.date.issued 2019-04-25
dc.identifier.issn 1471-2229
dc.identifier.uri http://library2.smu.ca/xmlui/handle/01/29945
dc.description Published Version en_CA
dc.description.abstract Elytrigia lolioides (Kar. et Kir.) Nevski, which is a perennial, cross-pollinating wheatgrass that is distributed in Russia and Kazakhstan, is classified into Elytrigia, Elymus, and Lophopyrum genera by taxonomists on the basis of different taxonomic classification systems. However, the genomic constitution of E. lolioides is still unknown. To identify the genome constitution and evolution of E. lolioides, we used single-copy nuclear genes acetyl-CoA carboxylase (Acc1) and elongation factor G (EF-G), multi-copy nuclear gene internal transcribed space (ITS), chloroplast gene trnL-F together with fluorescence and genomic in situ hybridization. Despite the widespread homogenization of ITS sequences, two distinct lineages (genera Pseudoroegneria and Hordeum) were identified. Acc1 and EF-G sequences suggested that in addition to Pseudoroegneria and Hordeum, unknown genome was the third potential donor of E. lolioides. Data from chloroplast DNA showed that Pseudoroegneria is the maternal donor of E. lolioides. Data from specific FISH marker for St genome indicated that E. lolioides has two sets of St genomes. Both genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) results confirmed the presence of Hordeum genome in this species. When E genome was used as the probe, no signal was found in 42 chromosomes. The E-like copy of Acc1 sequences was detected in E. lolioides possibly due to the introgression from E genome species. One of the H chromosomes in the accession W6-26586 from Kazakhstan did not hybridize H genome signals but had St genome signals on the pericentromeric regions in the two-color GISH. Phylogenetic and in situ hybridization indicated the presence of two sets of Pseudoroegneria and one set of Hordeum genome in E. lolioides. The genome formula of E. lolioides was designed as StStStStHH. E. lolioides may have originated through the hybridization between tetraploid Elymus (StH) and diploid Pseudoroegneria species. E and unknown genomes may participate in the speciation of E. lolioides through introgression. According to the genome classification system, E. lolioides should be transferred into Elymus L. and renamed as Elymus lolioidus (Kar. er Kir.) Meld. en_CA
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dc.description.provenance Made available in DSpace on 2021-09-29T16:15:39Z (GMT). No. of bitstreams: 1 Sun_Genlou_article_2019.pdf: 5604773 bytes, checksum: 088b8ea129ac53b58f741f1b678956c0 (MD5) Previous issue date: 2019-04-25 en
dc.language.iso en en_CA
dc.publisher Springer Nature en_CA
dc.relation.uri http://dx.doi.org/10.1186/s12870-019-1779-x
dc.rights © 2019 The Author(s). <a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a> which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.title Genome constitution and evolution of Elytrigia lolioides inferred from Acc1, EF-G, ITS, TrnL-F sequences and GISH en_CA
dc.type Text en_CA
dcterms.bibliographicCitation BMC Plant Biology 19(1), 158. (2019) en_CA
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© 2019 The Author(s). Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
 
Published Version: http://dx.doi.org/10.1186/s12870-019-1779-x
 
 

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