dc.creator |
Lu, Jie |
|
dc.creator |
Chang, Cheng |
|
dc.creator |
Zhang, Hai-Ping |
|
dc.creator |
Wang, Sheng-Xing |
|
dc.creator |
Sun, Genlou |
|
dc.creator |
Xiao, Shi-He |
|
dc.creator |
Ma, Chuan-Xi |
|
dc.date.accessioned |
2016-11-16T13:49:19Z |
|
dc.date.available |
2016-11-16T13:49:19Z |
|
dc.date.issued |
2015-12-14 |
|
dc.identifier.issn |
1932-6203 |
|
dc.identifier.uri |
http://library2.smu.ca/handle/01/26654 |
|
dc.description |
Publisher's Version/PDF |
en_CA |
dc.description.abstract |
Cytokinin oxidase (CKX) plays a crucial role in plant growth and development by reversibly inactivating cytokinin (CTK). Twenty-four primer pairs, designed from ESTs of the TaCKX genes family of common wheat, were used to identify their allelic variations associated with grain size, weight, and filling rate in 169 recombinant inbred lines (RIL) derived from Jing 411 × Hongmangchun 21. TaCKX6a02, a member of TaCKX gene family, amplified by primer pair T31–32, showed a close association with grain traits in this RIL population. Statistical analysis indicated that allelic variation of TaCKX6a02 had significant correlation with grain size, weight, and filling rate (GFR; P < 0.001) under varied environments. The TaCKX6a02-D1a allele from Jing411 significantly increased grain size, weight and grain filling rate, compared with TaCKX6a02-D1b from Hongmangchun 21. TaCKX6a02 was located on chromosome 3DS in the interval of Xbarc1119 and Xbarc1162, with a genetic distance of 1.4 cM. The location was further confirmed using Chinese Spring nulli–tetrasomic lines. A major QTL (quantitative trait locus) tightly linked to TaCKX6a02 was detected in the RIL population, explaining 17.1~38.2% of phenotype variations for grain size, weight, GFRmax and GFRmean in different environments. In addition, significant effects of variations of TaCKX6a02 on grain weight and GFR were further validated by association analysis among 102 wheat varieties in two cropping seasons. 12.8~35.1% of phenotypic variations were estimated for these genotypes. A novel 29-bp InDel behind the stop codon was detected by DNA sequence analysis between the two alleles of TaCKX6a02-D1. The gene-specific marker, TKX3D, was designed according to the novel variation, and can be used in marker-assisted selection (MAS) for grain size, weight, and GFR in common wheat. |
en_CA |
dc.description.provenance |
Submitted by John Yolkowski (john.yolkowski@smu.ca) on 2016-11-16T13:49:19Z
No. of bitstreams: 1
journal.pone.0144765.PDF: 3045024 bytes, checksum: 1c5ee86a0749e32c303eae1584944445 (MD5) |
en |
dc.description.provenance |
Made available in DSpace on 2016-11-16T13:49:19Z (GMT). No. of bitstreams: 1
journal.pone.0144765.PDF: 3045024 bytes, checksum: 1c5ee86a0749e32c303eae1584944445 (MD5)
Previous issue date: 2015-12-14 |
en |
dc.description.sponsorship |
This work was supported by grants from the National Natural Science Foundation of China (31000705), the China Agriculture Research System (CARS-03), the Scientific Research Special Program of Agricultural Commonwealth (201203033-04), Anhui province natural science foundation of China (1508085MC57), the Agriculture Research System of Anhui Province (AHCYTX-02) and the introduced leading talent research team for Universities in Anhui Province. The funders had no role in study design, |
en_CA |
dc.language.iso |
en |
en_CA |
dc.publisher |
Public Library of Science |
en_CA |
dc.relation.uri |
http://dx.doi.org/10.1371/journal.pone.0144765 |
|
dc.rights |
© 2015 Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
|
dc.subject.lcsh |
Wheat -- Genetics |
|
dc.subject.lcsh |
Wheat -- Growth |
|
dc.subject.lcsh |
Wheat -- Development |
|
dc.subject.lcsh |
Cytokinins |
|
dc.title |
Identification of a Novel Allele of TaCKX6a02 Associated with Grain Size, Filling Rate and Weight of Common Wheat |
en_CA |
dc.type |
Text |
en_CA |
dcterms.bibliographicCitation |
PLoS ONE 10(12), e0144765. (2015) |
en_CA |
Copyright statement:
© 2015 Lu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited