dc.contributor.advisor |
Brosseau, Christa L. |
|
dc.creator |
Davidson, Melanie Dawn |
|
dc.date.accessioned |
2019-05-16T14:52:50Z |
|
dc.date.available |
2019-05-16T14:52:50Z |
|
dc.date.issued |
2019 |
|
dc.identifier.other |
QD96 R34 D38 2019 |
|
dc.identifier.uri |
http://library2.smu.ca/handle/01/28590 |
|
dc.description |
xii, 74 leaves : illustrations (some colour) ; 29 cm |
|
dc.description |
Includes abstract and appendix. |
|
dc.description |
Includes bibliographical references (leaves 66-72). |
|
dc.description.abstract |
Multidimensional liquid chromatography (2D-LC) provides better resolving and separation power than conventional high-performance liquid chromatography (HPLC), and over the past decade has increasingly been applied in many different fields.<sup>1</sup> This thesis seeks to explore the extent to which surface-enhanced Raman spectroscopy (SERS) can be used as an offline detection modality for 2D-LC. This thesis hypothesizes that careful selection and modification of a three dimensional (3D) SERS substrate will be useful for characterization of fractions collected using 2D-LC. In particular, a mixture of four polyphenolic molecules was chosen for this proof-of-concept study. An optimised 2D-LC method was developed as part of this thesis. Various materials were evaluated as potential 3D-SERS substrates, with the most promising option being cellulose-based filter paper. Various modification strategies were explored to enhance the interaction between the polyphenolic molecules and the filter paper substrate. In the end, SERS-based detection of 2D-LC fractions proved challenging, even after optimization. |
en_CA |
dc.description.provenance |
Submitted by Greg Hilliard (greg.hilliard@smu.ca) on 2019-05-16T14:52:50Z
No. of bitstreams: 1
Davidson_Melanie_MASTERS_2019.pdf: 3167304 bytes, checksum: 49556c4fb18df44f7ece929afbe59029 (MD5) |
en |
dc.description.provenance |
Made available in DSpace on 2019-05-16T14:52:50Z (GMT). No. of bitstreams: 1
Davidson_Melanie_MASTERS_2019.pdf: 3167304 bytes, checksum: 49556c4fb18df44f7ece929afbe59029 (MD5)
Previous issue date: 2019-04-17 |
en |
dc.language.iso |
en |
en_CA |
dc.publisher |
Halifax, N.S. : Saint Mary's University |
|
dc.subject.lcc |
QD96.R34 |
|
dc.subject.lcsh |
Raman spectroscopy |
|
dc.subject.lcsh |
Raman effect, Surface enhanced |
|
dc.subject.lcsh |
Liquid chromatography |
|
dc.subject.lcsh |
Polyphenols |
|
dc.title |
An exploration of 2D-LC-SERS : a novel offline detection modality for multidimensional chromatography |
en_CA |
dc.type |
Text |
en_CA |
thesis.degree.name |
Master of Science in Applied Science |
|
thesis.degree.level |
Masters |
|
thesis.degree.discipline |
Chemistry |
|
thesis.degree.grantor |
Saint Mary's University (Halifax, N.S.) |
|