An in-depth investigation on the inhibitory activity of a native cave fungus against Pseudogymnoascus destructans

Show simple item record

dc.contributor.advisor Sit, Clarissa
dc.coverage.spatial Canada, Eastern
dc.creator Kolwich, Jennifer Lynn
dc.date.accessioned 2023-01-24T15:14:51Z
dc.date.available 2023-01-24T15:14:51Z
dc.date.issued 2021-08-20
dc.identifier.uri http://library2.smu.ca/xmlui/handle/01/31279
dc.description 1 online resource (ix, 111 pages) : colour illustrations, charts (some colour), graphs (some colour)
dc.description Includes abstract.
dc.description Includes bibliographical references (pages 107-111).
dc.description.abstract A metabolite-based treatment for <i>Pseudogymnoascus destructans</i>, the causative agent of bat White-nose syndrome, was isolated from a soil fungus native to Eastern Canada. Three application methods were modelled using media-based assays to determine how the treatment could be applied <i>in situ</i>: an environmentally-applied prophylactic, a topically applied prophylactic, and a topical post-infection treatment. <i>P. destructans</i> growth was effectively slowed in all three models.<br> After separation and characterisation, an active molecule in the metabolite-based treatment was determined to be griseofulvin, a known antifungal compound. This compound was tested against <i>P. destructans</i> using the topically-applied prophylactic method and proved to be more potent than the crude metabolite mixture.<br> To identify the mechanism of action of the metabolite-based treatment, transcriptome analysis was performed on control and treated groups of the target fungus <i>P. destructans</i> and a non-target Pseudogymnoascus species. To date, transcript reads were aligned and quantified. Further differential expression analysis must be completed. en_CA
dc.description.provenance Submitted by Greg Hilliard (greg.hilliard@smu.ca) on 2023-01-24T15:14:50Z No. of bitstreams: 1 Kolwich_Jennifer_MASTERS_2021.pdf: 8094530 bytes, checksum: 16338b2b692c82d443ca4660a6a7db2a (MD5) en
dc.description.provenance Made available in DSpace on 2023-01-24T15:14:51Z (GMT). No. of bitstreams: 1 Kolwich_Jennifer_MASTERS_2021.pdf: 8094530 bytes, checksum: 16338b2b692c82d443ca4660a6a7db2a (MD5) Previous issue date: 2021-08-20 en
dc.language.iso en en_CA
dc.publisher Halifax, N.S. : Saint Mary's University
dc.subject.lcsh White-nose syndrome -- Treatment
dc.subject.lcsh White-nose syndrome -- Prevention
dc.subject.lcsh Bats -- Ecology
dc.subject.lcsh Fungi -- Molecular aspects
dc.subject.lcsh Animal-fungus relationships
dc.subject.lcsh Metabolites -- Separation
dc.title An in-depth investigation on the inhibitory activity of a native cave fungus against Pseudogymnoascus destructans en_CA
dc.type Text en_CA
thesis.degree.name Master of Science in Applied Science
thesis.degree.level Masters
thesis.degree.discipline Chemistry
thesis.degree.grantor Saint Mary's University (Halifax, N.S.)
 Find Full text

Files in this item

 
 

This item appears in the following Collection(s)

Show simple item record